Analysis Pipeline

When you click "Run Analysis", EpiNexus works through up to six analysis steps. You can watch the progress bar in real time. Some steps (like super-enhancers and integration) are optional and can be enabled in your run settings.

01 Quality Control

~2 min

Checks whether your data is high quality — read mapping, signal enrichment, fragment sizes, and library complexity.

02 Annotation

~1 min

Labels each peak by where it sits in the genome (promoter, intron, intergenic, etc.) and finds the nearest gene.

03 Peak–Gene Linking

~1 min

Connects each enhancer or regulatory region to the gene(s) it most likely controls.

04 Differential

~3 min

Compares two conditions (e.g. treated vs. control) to find which peaks gained or lost signal.

05 Integration

~1 min

Combines multiple histone marks to classify regions as active, poised, bivalent, or repressed.

06 Super-Enhancers

~2 min

Identifies the large, high-signal regulatory regions that define cell identity and disease states. Enable this optional step in the run settings.

Supported Genomes

17 pre-configured genomes across all major model organisms. Add any other species via NCBI accession or your own reference files.

Human

hg38

Homo sapiens

GRCh38 · GENCODE v46

hg19

Homo sapiens

GRCh37 · GENCODE v46lift

Mouse

mm39

Mus musculus

GRCm39 · GENCODE vM35

mm10

Mus musculus

GRCm38 · GENCODE vM25

Rat

rn7

Rattus norvegicus

mRatBN7.2 · UCSC ncbiRefSeq

rn6

Rattus norvegicus

Rnor_6.0 · UCSC ncbiRefSeq

Zebrafish

danRer11

Danio rerio

GRCz11 · UCSC ncbiRefSeq

Invertebrates & Yeast

dm6

Drosophila melanogaster

BDGP6 · UCSC ncbiRefSeq

ce11

Caenorhabditis elegans

WBcel235 · UCSC ncbiRefSeq

sacCer3

Saccharomyces cerevisiae

R64 · UCSC ncbiRefSeq

Other Vertebrates

rheMac10

Macaca mulatta

Mmul_10 · Macaque

galGal6

Gallus gallus

GRCg6a · Chicken

xenLae2

Xenopus laevis

v10.1 · Frog

susScr11

Sus scrofa

Sscrofa11.1 · Pig

bosTau9

Bos taurus

ARS-UCD1.2 · Cow

canFam6

Canis lupus familiaris

UU_Cfam_GSD_1.0 · Dog

Plant

tair10

Arabidopsis thaliana

TAIR10 · Ensembl Plants 62

Any Other Organism

Custom Genome Support

EpiNexus works with any organism that has a reference genome. Add genomes from NCBI (over 1 million assemblies) or provide your own FASTA and GTF files.

Via NCBI accession

Any sequenced organism in the NCBI Assembly database

User-provided files

Custom assemblies, proprietary genomes, unpublished species

Reference data sources

Pre-configured genomes use FASTA sequences from UCSC and gene annotations from GENCODE (human and mouse), UCSC ncbiRefSeq (other animals), or Ensembl Plants (Arabidopsis). All references use chr-prefixed chromosome naming for consistency. Custom genomes can be added with a single command via the NCBI datasets CLI or from local files.